منابع مشابه
Mapping by Multiple Endonuclease Digestions
Construction of an accurate map of the sites where restriction endonucleases cleave DNA—restriction mapping—is critical for almost all subsequent manipulations of genetic material. Restriction mapping is based upon the cleavage of DNA at or near specific nucleotide sequences with restriction endonucleases and the determination of the sizes of the resulting DNA fragments by gel electrophoresis (...
متن کاملRapiGest SF Surfactant: An Enabling Tool for In-Solution Enzymatic Protein Digestions
The mechanism by which RapiGest SF improves the speed and completeness of digestion is illustrated in Figure 1. Mild protein denaturation opens protein structure and exposes the proteolytic sites to enzymatic cleavage. Enzymes are more resistant to denaturation than common proteins and remain active in RapiGest SF solutions. More complete denaturation of globular proteins can be accomplished by...
متن کاملComparison of non histone proteins selectively associated with nucleosomes with proteins released during limited DNase digestions.
Cultured mammary cells from GR mouse were used to analyse proteins associated with the mononucleosomes and released by a short micrococcal DNase treatment of nuclei. On metrizamide density gradients, mononucleosomes appear to be heterogeneous according to their content of associated non-histone proteins. Proteins associated with the denser fraction (1.22 - 1.24 g/ml) were analysed by two dimens...
متن کاملGlycoDigest: a tool for the targeted use of exoglycosidase digestions in glycan structure determination
UNLABELLED Sequencing oligosaccharides by exoglycosidases, either sequentially or in an array format, is a powerful tool to unambiguously determine the structure of complex N- and O-link glycans. Here, we introduce GlycoDigest, a tool that simulates exoglycosidase digestion, based on controlled rules acquired from expert knowledge and experimental evidence available in GlycoBase. The tool allow...
متن کاملIn vitro digestions and IgE binding of proteins from white and whole hen’s egg
Egg is leading the top 8 allergenic foods in infants. During gastrointestinal digestion, various factors can affect the proteolysis of food allergens, such as interaction with lipids or bile salts. Therefore, the amount of immunologically active protein reaching the intestinal mucosa can be substantially modified. Here we investigated egg white digestion under physiological conditions, includin...
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ژورنال
عنوان ژورنال: Revue d'anthropologie des connaissances
سال: 2020
ISSN: 1760-5393
DOI: 10.4000/rac.11042